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jelly derivedmsc c 12971  (PromoCell)


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    PromoCell jelly derivedmsc c 12971
    Jelly Derivedmsc C 12971, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+12971/hMSC-UC-c+Human+Mesenchymal+Stem+Cells/10__1088_slash_1748___605x_slash_adf1cd-90-1-5
    Average 94 stars, based on 56 article reviews
    jelly derivedmsc c 12971 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Neuro-muscular Regeneration Using Scaffolds with Mesenchymal Stem Cells (MSCs) Isolated from Human Umbilical Cord Wharton's Jelly: Functional and Morphological Analysis Using Rat Sciatic Nerve Neurotmesis Injury Model
    Article Snippet: .. Cell culture of MSCs from the Wharton’s jelly Our research group has been testing MSCs from the Promocell GmbH (C-12971, lot-number: 8082606.7) that are cultured and expanded in a humidified atmosphere with 5% CO2 at 37oC in Mesenchymal Stem Cell Medium, PromoCell (C-28010) [5,6,7]. .. To ensure that MSCs were not modified by the culture conditions, their phenotype is always confirmed by our research group immediately before in vivo testing by flow cytometry with the following antibodies and their respective isotypes (all from BioLegend unless stated otherwise): PE anti-human CD105 (eBioScience); APC anti-human CD73; PE anti-human CD90; PerCP/Cy5.5 anti-human CD45: FITC anti-human CD34; PerCP/Cy5.5 anti-human CD14; Pacific Blue anti-human CD19 and pacific-blue anti-human HLA-DR. Also cytogenetic analysis has been carried out before in vivo application between P4-P5 as previously described [5,6,7].

    In Vivo:

    Article Title: Long term performance evaluation of small-diameter vascular grafts based on polyvinyl alcohol hydrogel and dextran and MSCs-based therapies using the ovine pre-clinical animal model.
    Article Snippet: .. 2.2 MSCs culture and characterization before in vivo application Human MSCs isolated from the WJ of UC were purchased from PromoCell GmbH (C-12971, lot-number: 8082606.7). ..

    Article Title: Long term performance evaluation of small-diameter vascular grafts based on polyvinyl alcohol hydrogel and dextran and MSCs-based therapies using the ovine pre-clinical animal model.
    Article Snippet: .. MSCs culture and characterization before in vivo application Human MSCs isolated from the WJ of UC were purchased from PromoCell GmbH (C-12971, lot-number: 8082606.7). .. The MSCs were cultured and maintained in a humidified atmosphere with 5% CO2 at 37 C. Mesenchymal Stem Cell Medium, PromoCell (C28010) was replaced every 48 h. At 80% confluence, cells were harvested with 0.25% trypsin with EDTA (PubChem CID:6049), (GIBCO) and passed into a new flask for further expansion.

    Isolation:

    Article Title: Long term performance evaluation of small-diameter vascular grafts based on polyvinyl alcohol hydrogel and dextran and MSCs-based therapies using the ovine pre-clinical animal model.
    Article Snippet: .. 2.2 MSCs culture and characterization before in vivo application Human MSCs isolated from the WJ of UC were purchased from PromoCell GmbH (C-12971, lot-number: 8082606.7). ..

    Article Title: Long term performance evaluation of small-diameter vascular grafts based on polyvinyl alcohol hydrogel and dextran and MSCs-based therapies using the ovine pre-clinical animal model.
    Article Snippet: .. MSCs culture and characterization before in vivo application Human MSCs isolated from the WJ of UC were purchased from PromoCell GmbH (C-12971, lot-number: 8082606.7). .. The MSCs were cultured and maintained in a humidified atmosphere with 5% CO2 at 37 C. Mesenchymal Stem Cell Medium, PromoCell (C28010) was replaced every 48 h. At 80% confluence, cells were harvested with 0.25% trypsin with EDTA (PubChem CID:6049), (GIBCO) and passed into a new flask for further expansion.



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    Functional screening of drugs promoting chondrogenic differentiation ( A ) First Screening of Cartilage Differentiation-Promoting Drugs: Adipose-derived mesenchymal stem cells (ADMSCs) were treated with 10 µM of each drug for 72 h. The expression levels of COL2A1 (top) and SOX9 (bottom) mRNA were assessed using qRT-PCR. Dotted lines indicate drugs that significantly increased SOX9 and COL2A1 expression compared to DMSO control. ( B ) Second Screening: The Venn diagram shows the overlap of drugs that increased COL2A1 and SOX9 expression. Bar graphs present relative mRNA expression levels with statistical significance indicated. The cartilage-differentiation effect of seven drugs selected from the first screening was re-evaluated by measuring changes in mRNA levels of COL2A1, SOX9, and aggrecan (ACAN).: * P < 0.05, ** P < 0.01, *** P < 0.001 ( n = 3). ( C ) ADMSCs were cultured in pellet form and treated with DMSO, aripiprazole, or irinotecan for three weeks. Aripiprazole treatment showed increased cartilage differentiation compared to DMSO and irinotecan. ( D ) Hematoxylin and Eosin (H&E) staining and Alcian blue staining of pellet cultures. Aripiprazole treatment resulted in larger pellet size and more intense staining, indicating enhanced cartilage matrix production. Scale bars represent 100 μm.

    Journal: Scientific Reports

    Article Title: Therapeutic role of aripiprazole in cartilage defects explored through a drug repurposing approach

    doi: 10.1038/s41598-024-82177-1

    Figure Lengend Snippet: Functional screening of drugs promoting chondrogenic differentiation ( A ) First Screening of Cartilage Differentiation-Promoting Drugs: Adipose-derived mesenchymal stem cells (ADMSCs) were treated with 10 µM of each drug for 72 h. The expression levels of COL2A1 (top) and SOX9 (bottom) mRNA were assessed using qRT-PCR. Dotted lines indicate drugs that significantly increased SOX9 and COL2A1 expression compared to DMSO control. ( B ) Second Screening: The Venn diagram shows the overlap of drugs that increased COL2A1 and SOX9 expression. Bar graphs present relative mRNA expression levels with statistical significance indicated. The cartilage-differentiation effect of seven drugs selected from the first screening was re-evaluated by measuring changes in mRNA levels of COL2A1, SOX9, and aggrecan (ACAN).: * P < 0.05, ** P < 0.01, *** P < 0.001 ( n = 3). ( C ) ADMSCs were cultured in pellet form and treated with DMSO, aripiprazole, or irinotecan for three weeks. Aripiprazole treatment showed increased cartilage differentiation compared to DMSO and irinotecan. ( D ) Hematoxylin and Eosin (H&E) staining and Alcian blue staining of pellet cultures. Aripiprazole treatment resulted in larger pellet size and more intense staining, indicating enhanced cartilage matrix production. Scale bars represent 100 μm.

    Article Snippet: Human adipose tissue-derived mesenchymal stem cells (ADMSCs) were obtained from PromoCell GmbH (Heidelberg, Germany), ADMSCs were cultured in standard culture and chondrogenic differentiation media (Gibco).

    Techniques: Functional Assay, Derivative Assay, Expressing, Quantitative RT-PCR, Control, Cell Culture, Staining